Model Animal Competent Cell Technical Platform

[mindyhausler]

Animal cell culture medium is alkaline, and a small amount of phenol red indicator is often added to the medium. According to the color change of the indicator, the change of pH value of the medium can be displayed directly. At the beginning of culture, the pH value of the culture medium was 7.4. In the process of culture, because more CO2 and lactic acid are produced with the increase of cell concentration, the pH value will decrease, but it must not be lower than pH 7.0. It is very important to accurately control the pH value, which is generally 6.7-6.9, and its fluctuation range is 0.05-0.9. Although the optimal pH value of many hybridoma cells is about 7.0, when it decreases to 6.8, it will inhibit the growth.

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